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目的：研究胃癌ｐ５３基因表达以及与ＤＮＡ含量和细胞增殖活性的关系。方法：采用流式细胞术和细胞免疫荧光技术检测胃癌ｐ５３蛋白、ＤＮＡ和细胞周期。结果：５６例胃癌ｐ５３蛋白阳性３２例（５７．１２％），正常胃粘膜和良性病变组均为阴性。ｐ５３蛋白阳性者多为异倍体肿瘤，ＤＩ值、ＳＰＦ和ＰＩ值均明显高于阴性者，两组差异显著（Ｐ＜０．０１）。结论：ｐ５３基因突变可能是促进胃癌发生发展和导致肿瘤细胞增殖异常活跃的重要因素。
Objective: To investigate the expression of p53 gene in gastric cancer and its relationship with DNA content and cell proliferation activity. Methods: Flow cytometry and cell immunofluorescence techniques were used to detect gastric cancer p53 protein, DNA and cell cycle. Results: There were 32 cases (57.12%) of p53 protein positive in gastric cancer. The normal gastric mucosa and benign lesions were negative. Most of the patients with positive p53 protein were heteroploid tumors. The DI, SPF, and PI values were significantly higher than those negative. There was a significant difference between the two groups (P<0.01). Conclusion: p53 gene mutation may be an important factor in promoting the occurrence and development of gastric cancer and causing abnormal proliferation of tumor cells.