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目的:探讨薯蓣皂苷元(diosgenin,DG)对H2O2诱导损伤的PC12细胞的保护作用。方法:用H2O2损伤PC12细胞建立氧化应激损伤细胞模型,在电子显微镜下观察其细胞形态学特征,并通过MTT法检测不同浓度DG对正常PC12细胞和模型细胞增殖的影响;应用流式细胞术检测DG对模型细胞凋亡率和细胞周期的影响。结果:与正常组比较,不同浓度H2O2(100、200、400μmol/L)损伤组PC12细胞生存率均显著下降(P<0.01),DG 3个浓度组(1.25、2.5、5.0μmol/L)PC12细胞生存率均显著提高(P<0.01)。与模型组比较,DG 3个浓度组(1.25、2.5、5.0μmol/L)PC12细胞生存率均显著提高(P<0.01);2.5μmol/L的DG可显著抑制H2O2诱导的PC12细胞凋亡(P<0.01),在细胞周期上表现为G0~G1期细胞减少,同时S期增多。结论:DG能促进PC12细胞增殖,对H2O2氧化损伤的PC12细胞具有保护作用。
Objective: To investigate the protective effect of diosgenin (DG) on PC12 cells induced by H2O2. Methods: The oxidative stress injury model was induced by H2O2 injury in PC12 cells. Morphological characteristics of cells were observed under electron microscope. The effects of different concentrations of DG on the proliferation of normal PC12 cells and model cells were detected by MTT assay. Flow cytometry The effect of DG on apoptosis rate and cell cycle of model cells was examined. Results: Compared with the normal group, the survival rates of PC12 cells were significantly decreased in H2O2-treated groups (100, 200, 400μmol / L) (P <0.01) Cell survival rates were significantly increased (P <0.01). Compared with the model group, the survival rates of PC12 cells in DG 3 concentration group (1.25,2.5,5.0μmol / L) were significantly increased (P <0.01); 2.5μmol / L DG could significantly inhibit the apoptosis of PC12 cells induced by H2O2 P <0.01), the cell cycle showed G0 ~ G1 cells decreased, while S increased. Conclusion: DG can promote the proliferation of PC12 cells and protect the PC12 cells from oxidative damage by H2O2.