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目的:探讨鼠原性内皮抑素(ES)联合小剂量阿霉素治疗裸鼠乳腺癌的疗效。方法:构建逆转录病毒载体质粒DFG-ESDNA,建立乳腺癌裸鼠模型,将肿瘤生长均匀的裸鼠分成对照组、ES组、小剂量阿霉素组、ES与小剂量阿霉素联合治疗组。分别于肿瘤对侧背部皮下注射1×10~6(0.1 mL)转染ES的人成纤维细胞,尾静脉注射阿霉素1 mg/kg。每周测量1次肿瘤大小,根据V=0.52×12×W计算体积。结果:ES与小剂量阿霉素联合治疗组肿瘤体积明显小于其他各组(P<0.01),细胞凋亡率明显高于其他各组(P<0.01)。结论:ES可以有效抑制肿瘤血管生成,抑制裸鼠乳腺癌的生长,与小剂量阿霉素联合应用可明显增强其抗乳腺癌生长的疗效,为今后探索乳腺癌治疗提供新途径。
Objective: To investigate the therapeutic effect of murine endostatin (ES) combined with low dose of doxorubicin on breast cancer in nude mice. Methods: The retrovirus vector plasmid DFG-ESDNA was constructed and the nude mice model of breast cancer was established. The nude mice with tumor growth were divided into control group, ES group, low-dose doxorubicin group, ES and low-dose doxorubicin combined treatment group . Human fibroblasts were transfected with 1 × 10 ~ 6 (0.1 mL) ES into the contralateral dorsum of the tumor, respectively, and doxorubicin 1 mg / kg was injected into the caudal vein. Tumor size was measured weekly and volume was calculated according to V = 0.52 × 12 × W. Results: The tumor volume of ES combined with low dose doxorubicin was significantly less than that of other groups (P <0.01), and the apoptosis rate was significantly higher than that of other groups (P <0.01). Conclusion: ES can effectively inhibit tumor angiogenesis and inhibit the growth of breast cancer in nude mice. Combined with low dose of doxorubicin can significantly enhance its anti-tumor effect and provide a new way to explore breast cancer treatment in the future.