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以榨菜根肿病发病程度不同的根际土壤为分离对象,采用454焦磷酸高通量测序方法,研究了榨菜根肿病对根际土壤真菌群落的影响,并对榨菜根际土壤真菌的18SrDNA序列进行大规模测序,揭示根肿病不同程度发病田根际土壤真菌群落的变化特征。结果表明:土壤中子囊菌为优势真菌类群,其次为担子菌;重病田土壤中植物病原真菌数量高于其它发病田;重病田土壤真菌群落的Shannon和Simpson多样性指数、OTU数量和属的数量均低于其它发病田;表明榨菜根肿病在一定程度上影响根际土壤真菌群落的结构,这可能与根肿菌造成榨菜根系破坏后,其根系分泌物旺盛有关。
The rhizosphere soil with different degrees of clubroot disease was used as the target. The 454 pyrophosphate high-throughput sequencing method was used to study the effects of tuberous root clubbing on rhizosphere soil fungal community. The effects of 18S rDNA Sequence of large-scale sequencing to reveal different degrees of clubroot rhizosphere soil fungal community changes. The results showed that the ascosphere in soil was the dominant fungi, followed by Basidiomycetes. The number of phytopathogenic fungi in the seriously ill soil was higher than that in other fields. The Shannon and Simpson index, OTU and the genus All the results showed that clubroot clubhess affected the structure of fungal community in rhizosphere soil to a certain extent, which may be related to the excretion of root exudates after root tubercle root destruction.