论文部分内容阅读
本文以甘蔗‘粤糖94/128’(YT 94/128)和‘桂糖37号’(GT 37)作为研究材料,采用针刺接种法,分析了不同甘蔗在梢腐病菌侵染下氮代谢相关指标的变化。结果表明,未接种状态下,YT 94/128叶片的核糖核酸(RNA)、可溶性蛋白(SP)、硝态氮(NO_3~--N)、铵态氮(NH_4~+-N)含量和叶片硝酸还原酶(NR)、谷氨酰氨合成酶(GS)、谷氨酸合成酶(GOGAT)活性都要高于GT37,且都表现出稳中有升的变化规律。受到梢腐病菌侵染后,GT 37叶片RNA含量显著下降,GS、GOGAT活性以及SP含量下降速度显著快于YT 94/128。数据显示两类材料中氮代谢各类指标存在不同程度相关性,也存在氨基酸组分差异。由此可见,梢腐病菌侵染可直接导致甘蔗叶片RNA降解,NR、GS以及GOGAT活性下降,SP下降,游离氨基酸(AA)总量上升。氨基酸不同组分与甘蔗建立抗梢腐病菌侵染机制密切相关。
In this paper, sugarcane 'Yueshun 94/128' (YT 94/128) and 'Guitang 37' (GT 37) were used as materials to study the effects of different sugar cane on nitrogen metabolism Changes in relevant indicators. The results showed that the contents of RNA, soluble protein (SP), nitrate nitrogen (NO_3 - N) and ammonium nitrogen (NH_4 ~ + -N) in leaves of YT 94/128 and leaves Nitrate reductase (NR), glutamine synthetase (GS) and glutamic acid synthase (GOGAT) activity were higher than that of GT37, and all showed a stable variation. The RNA content of GT 37 leaves significantly decreased after inoculation with P. grisea. The activities of GS, GOGAT and SP decreased significantly faster than that of YT 94/128. The data show that there are different degrees of correlation between various indexes of nitrogen metabolism in the two types of materials, and there are also differences in amino acid components. It can be seen from the above results that RNAi infection could directly cause RNA degradation of sugarcane leaves, decrease of NR, GS and GOGAT activity, decrease of SP and increase of total free amino acids (AA). Different components of amino acids are closely related to the establishment of infection mechanism of sugarcane.