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目的:检测神经突蛋白(neuritin)在大鼠创伤性脑损伤组织中的时相性表达,探讨其在脑损伤后的意义。方法:42只SD大鼠分为正常组、对照组和实验组,改良Feeney法建立大鼠脑损伤模型,术后分6 h、12 h、24 h、3 d、7 d、14 d共6个亚组。免疫组化、Western-blot检测脑损伤组织中neuritin蛋白表达。结果:免疫组化:实验组neuritin蛋白6 h呈阳性表达,12 h~7 d呈强阳性,明显高于对照组与正常组,14 d呈弱阳性表达。Western-blot:实验组neuritin蛋白6 h稍增高,24 h达高峰,持续到7 d,与对照组、正常组比较差异均有统计学差异(P<0.01),14 d明显降低,较正常组差异仍有统计学意义(P<0.05)。结论:Neuritin蛋白在脑损伤组织中表达明显增高,可能在脑损伤后起着重要作用。
Objective: To detect the temporal expression of neuritin in traumatic brain injury in rats and to explore its significance after brain injury. Methods: Forty-two SD rats were divided into normal group, control group and experimental group. The brain injury model was established by modified Feeney method. The rats were divided into 6 hours, 12 hours, 24 hours, 3 days, 7 days and 14 days Subgroups. Immunohistochemistry and Western-blot were used to detect the expression of neuritin in brain tissue. Results: Immunohistochemistry: Neuritin protein in the experimental group was positive for 6 h, strongly positive for 12 h to 7 d, significantly higher than that in the control group and the normal group, and weakly positive on the 14th day. Western-blot: The neuritin protein in the experimental group increased slightly at 6 h and peaked at 24 h, reaching the level of 7 d, showing significant difference compared with the control group and the normal group (P <0.01), and significantly decreased at 14 d The difference was still statistically significant (P <0.05). Conclusion: Neuritin protein is significantly increased in brain injury and may play an important role in brain injury.