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采用分步提取的方式从中药箬叶中分离得到8种多糖组分:酸性杂多糖FS、FE、FⅠ,β-D-葡萄糖醛酸聚糖FⅡ和四种半纤维素多糖α-D-木聚糖FⅢ-a、FⅢ-b、FⅣ-a及FⅣ-b.紫外光谱、红外光谱、凝胶色谱、元素分析等结果表明8种箬叶多糖为纯品.并采用纸层析,气相色谱分析确定其单糖组成.采用高效凝胶渗透色谱GPC法测定了4种箬叶多糖FE、FⅠ、FⅢ-a及FⅣ-a的重均分子量Mw、数均分子量Mn,均为大分子,分子量分布较窄,纯度较高.
Eight fractions of polysaccharides were isolated from the leaves of Chinese traditional medicine by fractional extraction: acidic heteropolysaccharide FS, FE, FⅠ, β-D-glucuronosyl FⅡ and four hemicellulose polysaccharides α-D-wood Glycan FⅢ-a, FⅢ-b, FⅣ-a and FⅣ-b. UV spectroscopy, infrared spectroscopy, gel chromatography, elemental analysis and other results showed that 8 kinds of leaf polysaccharide as pure. And the use of paper chromatography, gas chromatography to determine the monosaccharide composition. The weight average molecular weights Mw and number average molecular weights Mn of four kinds of polysaccharides FE, FⅠ, FⅢ-a and FⅣ-a were determined by high performance gel permeation chromatography (GPC). All of them were macromolecules with narrow molecular weight distribution and high purity .