论文部分内容阅读
以玉米花丝为材料,采用苯胺蓝染色法检测授粉后玉米花粉管的伸长进程。授粉0.5 h后开始每隔1 h对花丝取样,直至48 h;所取花丝经固定、软化和苯胺蓝染色后压片,在倒置荧光显微镜紫外光滤片下观测花粉管伸长情况。结果表明,花粉在授粉后0.5 h就开始萌发,随后花粉管开始伸长并逐渐加速,3 h后以较高速度0.8~0.9 cm/h伸长,维持17 h后速度开始下降,22 h后大部分进入子房,整个过程中花粉管的平均伸长速度为0.68 cm/h。玉米花粉离体自然条件下的生命时间为5 h,此后开始分解。在玉米花粉介导转基因时需对离体的花粉与外源基因进行处理,这个过程消耗花粉部分内容物和能量,且影响花粉的活力,是玉米花粉介导法转化率偏低的一个重要原因。
Using corn silk as material, the elongation of pollen tube after pollination was detected by aniline blue staining. At 0.5 h after pollination, the filament was sampled every 1 h until 48 h. The obtained filament was fixed, softened and stained with aniline blue. The elongation of pollen tube was observed under an inverted fluorescence microscope (UV). The results showed that pollen began to germinate 0.5 h after pollination, then the pollen tube began to elongate and gradually accelerated. After 3 h, the pollen tube was elongated at a higher speed of 0.8-0.9 cm / h, and the speed began to decline after 17 h. After 22 h Most into the ovary, the average pollen tube elongation rate of 0.68 cm / h. Corn pollen in vitro life of natural conditions for 5 h, then began to decompose. Pollen and exogenous genes need to be manipulated in vitro when the pollen is mediated by maize pollen. This process consumes part of the pollen content and energy and affects pollen viability, which is an important reason for the low pollen-mediated transformation rate .