论文部分内容阅读
目的探讨Livin、Survivin、P16、34βE12在预测膀胱低度恶性潜能泌尿上皮乳头状肿瘤(PUNLMP)复发中的临床意义。方法回顾性分析31例PUNLMP标本,采用免疫组织化学Envision法检测PUNLMP组织中Livin、Survivin、P16、34βE12的表达情况。结果 Livin、Survivin、P16、34βE12在PUNLMP中的高表达率分别为51.61%、74.19%、64.52%、87.10%。随访时间12~49个月不等,中位随访时间34.00个月,在随访中发现P16和Livin的表达差异在预测PUNLMP的术后复发方面存在统计学意义(χ2分别=10.11、19.61,P均<0.05),34βE12、Survivin的表达差异在预测PUNLMP的术后复发方面无统计学意义(χ2分别=0.00、0.77,P均>0.05)。P16的表达情况与肿瘤的长径大小有关(χ2=5.84,P<0.05),P16异常表达与Livin呈负相关(r=-0.46,P<0.05),膀胱内灌注化疗能够延长PUNLMP患者术后复发时间。结论联合检测Livin与P16的表达,对预测PUNLMP的复发有较高的临床意义。所有PUNLMP患者术后均需行膀胱灌注化疗,而对检测出Livin呈高表达,且伴有P16呈低表达的PUNLMP患者术后应该追加化疗次数,延长化疗时间。
Objective To investigate the clinical significance of Livin, Survivin, P16 and 34βE12 in predicting the recurrence of bladder low grade malignant potential urothelial papilloma (PUNLMP). Methods 31 PUNLMP specimens were retrospectively analyzed. The expression of Livin, Survivin, P16 and 34βE12 in PUNLMP tissues was detected by immunohistochemistry Envision method. Results The high expression rates of Livin, Survivin, P16 and 34βE12 in PUNLMP were 51.61%, 74.19%, 64.52% and 87.10% respectively. The follow-up time ranged from 12 to 49 months, and the median follow-up time was 34.00 months. There was a significant difference in the expression of P16 and Livin between the two groups (χ2 = 10.11, 19.61, P < <0.05). There was no significant difference in the expression of 34βE12 and Survivin in predicting postoperative recurrence of PUNLMP (χ2 = 0.00,0.77, P> 0.05). The expression of P16 was related to the major diameter of tumor (χ2 = 5.84, P <0.05). The abnormal expression of P16 was negatively correlated with Livin (r = -0.46, P <0.05). Intrapleural chemotherapy could prolong the postoperative duration of PUNLMP Recurrence time. Conclusion Combined detection of Livin and P16 expression has a high clinical value in predicting the recurrence of PUNLMP. All patients with PUNLMP require intravesical chemotherapy after bladder irrigation. Patients with PUNLMP with high expression of Livin and low P16 expression should be given additional chemotherapy and prolonged chemotherapy.