作者采用HPLC方法可专一定量测定人体唾液中的舒缓激肽(Bradykinin,BK),而无需使用复杂的解剖技术或其它生物反应,以期说明BK是牙周炎的化学介质。样品制备:将牙周炎患者的唾液收集于冰冷的聚乙烯管中,立即用超滤膜过滤(Immersible CX—10),最好加入酶抑制剂以免BK被唾液中激肽酶降解或合 Using the HPLC method, the authors are able to quantify Bradykinin (BK) in human saliva quantitatively without the need for complex anatomical techniques or other biological reactions to demonstrate that BK is the chemical mediator of periodontitis. Sample Preparation: Saliva from patients with periodontitis was collected in an ice-cold polyethylene tube and immediately filtered through an ultrafiltration membrane (Immersible CX-10), preferably with an enzyme inhibitor to prevent degradation of BK by kinases in saliva or