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正常免疫球蛋白IgG药剂是已知含有对细胞因子白介素(IL)-1α,IL-6和干扰素(IFN)α高度亲合力中和抗体(Ab)的。为了试验其它细胞因子Ab,用饱和结合8种碘~(125)标记的重组细胞因子对23批IgG进行了实验,所有批白细胞-巨噬细胞集落刺激因子(GM-CSF)均以高亲和力(约10pmol/L)和容量(高达5mumol Gm-CSF/mol IgG)结合,仅1/15批以高亲和力结合IL-5,而有13/15批以较低容量和亲和力结合IL-10。没有一批IgG制剂是结合IL-1受体拮抗物,IL-2,IL-3,IL-4和G-CSF的。交叉结合和吸附分析揭示,与重组GM-CSF,IL-5和IL-10的结合较其天然者具有同样的或略强的结合力。GM-
Normal immunoglobulin IgG agents are known to contain a high affinity neutralizing antibody (Ab) to the cytokines interleukin (IL) -1α, IL-6 and interferon (IFN) α. To test other cytokines Ab, 23 batches of IgG were tested by saturation with 8 types of iodine-125 labeled recombinant cytokines and all of the batch white blood cell-macrophage colony stimulating factor (GM-CSF) About 10 pmol / L) and capacity (up to 5 mumol Gm-CSF / mol IgG), only 1/15 batches bind IL-5 with high affinity while 13/15 batches bind IL-10 with lower capacity and affinity. None of the IgG preparations bind to IL-1 receptor antagonists, IL-2, IL-3, IL-4 and G-CSF. Cross-binding and adsorption analysis revealed that the binding to recombinant GM-CSF, IL-5 and IL-10 had the same or slightly stronger binding than their native counterparts. GM-