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探讨海湾扇贝多肽(polypeptide from bay scallop,PBS)对小鼠H22肝癌移植瘤的抑瘤作用及机制。将50只KM小鼠随机分为正常对照组、H22肝癌模型组和PBS低、中、高剂量组共5组,按试验设计进行体内灌胃试验。结果显示,低、中、高剂量组的抑瘤率分别为28.16%、41.93%和84.00%,具有量效依赖趋势。与模型组相比,三剂量组小鼠血清中突变型p53、Survivin、8-iso-PGF2α和MDA的水平均有所降低,而IL-2、GSH-Px、SOD和TNF-α的水平均有所升高,且中剂量组较其他两组效果更明显;三剂量组小鼠肝脏组织提取液呈现相同变化趋势。肿瘤组织病理学观察也表明PBS有较好的抗肿瘤生物学效应。PBS对小鼠肝癌H22细胞具有明显的抑制作用。
To investigate the antitumor effect and mechanism of polypeptide from bay scallop (PBS) on mouse hepatoma H22 transplanted tumor. 50 KM mice were randomly divided into normal control group, H22 liver cancer model group and PBS low, medium and high dose group of 5 groups, according to the experimental design for in vivo gavage. The results showed that the tumor inhibition rates of low, medium and high dose groups were 28.16%, 41.93% and 84.00%, respectively, with dose-dependent trend. Compared with the model group, the serum levels of mutant p53, Survivin, 8-iso-PGF2α and MDA in the three dose groups were decreased, while the levels of IL-2, GSH-Px, SOD and TNF- Increased, and the effect of the middle dose group is more obvious than the other two groups; the three dose group mice liver tissue extract showed the same trend. Tumor histopathology also showed that PBS has good anti-tumor biological effects. PBS on mouse hepatoma H22 cells had a significant inhibitory effect.