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目的探讨不同浓度的瘦素对5-氟尿嘧啶(5-FU)损伤结肠癌细胞的影响。方法同时用5-FU及不同浓度的瘦素进行体外干预结肠癌HT-29细胞株。MTT法检测5-FU50%细胞生长抑制率(IC50)的变化。流式细胞仪、原位末端转移酶标记法(TUNEL)进行周期及凋亡分析。以RT-PCR方法检测caspase-9,caspase-3mRNA的表达。结果瘦素抑制5-FU对HT-29的杀伤作用。抑制5-FU诱导的细胞周期阻滞及细胞凋亡。RT-PCR表明加入瘦素后caspase-9,caspase-3mRNA的表达均下降,且呈剂量依赖性。结论瘦素通过下调caspase-9,caspase-3的表达促进结肠癌细胞产生凋亡抵抗,抑制5-FU的损伤作用。
Objective To investigate the effects of different concentrations of leptin on 5-fluorouracil (5-FU) -induced colon cancer cells. Methods Human colon cancer HT-29 cells were treated with 5-FU and different concentrations of leptin in vitro. MTT assay 5-FU50% cell growth inhibition (IC50) changes. Flow Cytometry and TUNEL were used for cycle and apoptosis analysis. The expression of caspase-9 and caspase-3 mRNA was detected by RT-PCR. Results Leptin inhibited the killing effect of 5-FU on HT-29. Inhibit 5-FU-induced cell cycle arrest and apoptosis. RT-PCR showed that the expression of caspase-9 and caspase-3 mRNA decreased after addition of leptin in a dose-dependent manner. Conclusion Leptin can promote the apoptosis resistance of colon cancer cells by inhibiting the expression of caspase-9 and caspase-3, and inhibit the injury of 5-FU.