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建立了一种基于Fe~(3+)-方酸螯合体系的葡萄糖可见光谱分析方法。在葡萄糖氧化酶(GOx)的催化作用下,葡萄糖被氧化为葡萄糖酸和H_2O_2,产生的H_2O_2将Fe~(2+)氧化为Fe~(3+),Fe~(3+)与方酸的螯合体系可产生灵敏的显色反应,从而建立了葡萄糖的检测方法。结果表明,所构建的葡萄糖传感器,葡萄糖在0.01~1.0 mmol/L的浓度范围内有很好的线性关系(R=0.9962),检测限为5μmol/L。与市售血糖仪相比,表现出更高的灵敏度,且在8 h内有很好的稳定性。方法可用于胎牛血清样品中葡萄糖的检测。
A method based on Fe ~ (3 +) - Squaric acid chelate system for the determination of glucose was established. Under the action of glucose oxidase (GOx), glucose is oxidized to gluconic acid and H 2 O 2, and the produced H 2 O 2 oxidizes Fe 2+ to Fe 3+, Fe 3+ and squalic acid Chelation system can produce a sensitive color reaction, thus establishing a glucose detection method. The results showed that there was a good linearity (R = 0.9962) between glucose sensor and glucose in the range of 0.01-1.0 mmol / L with a detection limit of 5 μmol / L. Compared with the commercial blood glucose meter, showed higher sensitivity, and in 8 h has good stability. The method can be used for the detection of glucose in fetal bovine serum samples.