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肿瘤的原发与继发性多药耐药已成为临床成功治疗肿瘤的巨大障碍，而这种多药耐药已证明与MDR基因的表达成正相关。根据PCR工作原理设计一种以标准MDR1DNA为参照的PCR定量检测肿瘤组织MDR1mRNA的方法。同时对10例胃癌、6例大肠癌及4例非肿瘤胃组织标本进行了检测。结果有4例MDR1mRNA高表达，其中胃癌3例，大肠癌1例，其余癌组织及非肿瘤胃组织均未检出。3例胃癌均为低分化腺癌，占被检低分化胄腺癌的60％（3／5），大肠癌1例为中分化腺癌。因此，肿瘤组织的MDR1mRNA表达似乎与其组织学类型有关，低分化癌MOR1mRNA呈高表达倾向，这也有可能是低分化癌往往水后化疗不满意的原因之一。
The primary and secondary multidrug resistance of tumors has become a huge obstacle to the successful clinical treatment of tumors, and this multidrug resistance has been shown to be positively correlated with the expression of MDR genes. According to the working principle of PCR, a method for quantitative detection of MDR1 mRNA in tumor tissues using standard MDR1 DNA as a reference was designed. At the same time, 10 cases of gastric cancer, 6 cases of colorectal cancer and 4 cases of non-tumor gastric tissue were detected. Results There were 4 cases of high expression of MDR1 mRNA, including 3 cases of gastric cancer and 1 case of colorectal cancer. No other cancer tissues and non-tumor gastric tissues were detected. Three cases of gastric cancer were poorly differentiated adenocarcinoma, accounting for 60% (3/5) of poorly differentiated adenocarcinomas. One case of colorectal cancer was moderately differentiated adenocarcinoma. Therefore, the expression of MDR1 mRNA in tumor tissues seems to be related to histological type. MOR1 mRNA is highly expressed in poorly differentiated cancers, which may be one of the reasons why poorly differentiated cancer is often unsatisfactory after water chemotherapy.