论文部分内容阅读
用ＰＣＲ、ＧＵＳ染色和Ｗｅｓｔｅｒｎ点杂交技术检测了Ｂｔ水稻杂交后代群体，在３９４株ＧＵＳ阳性 株中，有３９２株表达Ｂｔ蛋白，协同表达株率达９９．４９％。由此表明，在杂交后代中报告基因ｇｕｓ和 目的基因ｃｒｙ１Ａｂ紧密连锁遗传与表达。ＧＵＳ组织染色和Ｓｏｕｔｈｅｒｎ杂交检测表明Ｂｔ水稻中的 ＣｒｙｌＡｂ基因呈单位点显性基因遗传，且在有性世代中能稳定传递。还发现，在ＢＣ１、ＢＣ１Ｆ２和粳粳 交Ｆ２群体中ＣｒｙｌＡｂ基因呈单位点显性基因遗传，而在籼粳交Ｆ２群体中偏离３∶１分离。
The populations of Bt hybrids were detected by PCR, GUS staining and Western blotting. Among 394 GUS positive strains, 392 strains expressed Bt protein, and the synergistic expression rate was 99.49%. This indicated that in the hybrid progeny reporter gene gus and the target gene cry1Ab closely linked genetic and expression. The results of GUS staining and Southern hybridization showed that the CrylAb gene in Bt rice was inherited as a unit dominant gene and could be transmitted stably in the sexual generation. It was also found that the CrylAb gene was inherited as a unit dominant gene in the F2 population of BC1, BC1F2 and japonica-japonica crosses, and segregated 3: 1 in F2 population of indica-japonica crosses.