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目的初步探讨趋化因子基质细胞衍生因子-1α(SDF-1α)及其受体CXCR4在有血液系统损害的系统性红斑狼疮(SLE)患者外周血的表达水平及意义。方法用流式细胞术分别检测20名健康对照者、15例SLE非血液系统损害患者,25例SLE血液系统损害患者外周血中CD3+CD4+T淋巴细胞、CD3+CD8+T淋巴细胞表面CXCR4的表达百分率,采用双抗体夹心ABC-ELISA法检测以上各组血清SDF-1α浓度水平。结果健康对照者外周血中CD3+CD4+T淋巴细胞、CD3+CD8+T淋巴细胞表面CXCR4表达百分率及血清SDF-1α浓度水平均明显低于SLE非血液系统损害患者和SLE血液系统损害患者(P<0.05),而SLE非血液系统损害患者外周血中CD3+CD4+T淋巴细胞、CD3+CD8+T淋巴细胞表面CXCR4表达百分率及血清SDF-1α浓度水平也均明显低于SLE血液系统损害患者(P=0.000)。SLE血白细胞减少患者、血小板减少患者、贫血患者的外周血中SDF-1α、CXCR4表达水平也较SLE非血白细胞减少患者、血小板减少患者、贫血患者高。结论 SDF-1α、CXCR4可能在SLE血液系统损害的发病过程中起重要作用。
Objective To investigate the expression of chemokine stromal cell-derived factor-1α (SDF-1α) and its receptor CXCR4 in peripheral blood of systemic lupus erythematosus (SLE) patients with blood system damage. Methods Flow cytometry was used to detect the expression of CXCR4 in 20 healthy controls, 15 patients with SLE non-hematologic damage, 25 CD3 + CD4 + T lymphocytes in peripheral blood and 25 CD3 + CD8 + T lymphocytes in patients with SLE hematological injury Of the expression of the percentage of double antibody sandwich ABC-ELISA method to detect serum SDF-1α levels in the above groups. Results The percentages of CXCR4 expression on the surface of CD3 + CD4 + T lymphocytes and CD3 + CD8 + T lymphocytes in peripheral blood of healthy controls were significantly lower than those of patients with SLE non-hematologic damage and those with SLE hematological damage P <0.05). However, the expression of CXCR4 on the surface of CD3 + CD4 + T lymphocytes and CD3 + CD8 + T lymphocytes in peripheral blood of patients with SLE non-hematologic damage were also significantly lower than that of SLE hematological system Patients (P = 0.000). SLE leukopenia patients, thrombocytopenia, anemia patients with peripheral blood levels of SDF-1α, CXCR4 expression than SLE non-leukopenia, thrombocytopenia patients, anemia patients. Conclusion SDF-1α and CXCR4 may play an important role in the pathogenesis of SLE blood system damage.