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以平邑甜茶(Malus hupehensis Rehd.)幼苗为试材,在连作土盆栽条件下探讨了内生真菌球毛壳(Chaetomium globosmn)ND35菌肥对其生长和土壤环境的影响。试验共设3个处理:空白对照、连作土壤施加2%菌糠和连作土壤中施加2%球毛壳菌ND35菌肥(即菌糠+ND35菌株)。结果表明:与对照相比,施加2%菌糠和ND35菌肥对平邑甜茶植株的生物量均有不同程度的促进作用,其中菌肥效果最为明显,施加2%菌肥的7月植株干、鲜样质量分别增加了71.9%、71.6%,9月增加了155.9%、153.1%,9月施加2%菌肥的效果比菌糠更加明显。施加2%菌糠和ND35菌肥均增加了土壤中微生物数量,且随着处理时间的增长,土壤细菌和放线菌数量均呈增加趋势,9月最多。其中施加2%菌肥增幅最高,7、8、9月细菌数量分别比对照增加了245.9%、89.1%、216.3%,放线菌数量分别比对照增加了68.7%、124.7%、131.1%。两种处理均增加了土壤酶的活性,8月的土壤脲酶、蔗糖酶和磷酸酶活性最高,其中以施加2%菌肥的土壤酶活性增幅最为明显。施加2%菌肥还增加了平邑甜茶植株的根系活力,7、8、9月分别比对照增加了59.0%、50.2%、20.2%。与对照和施加菌糠处理相比,球毛壳ND35菌肥可以更好地改善苹果连作土壤微生物区系(microflora),提高土壤酶活性,增加平邑甜茶幼苗根系活力和生物量。
Using the seedlings of Malus hupehensis Rehd. As test material, the effects of endophytic fungus Chaetomium globosmn ND35 on its growth and soil environment were studied under potted condition. In the experiment, there were 3 treatments: blank control, 2% fungi bran in continuous cropping soil and 2% Chaetomium rhodochrous ND35 (ie fungus bran + ND35 strain) in continuous cropping soil. The results showed that compared with the control, the application of 2% bran and ND35 could promote the biomass of P. xylophilus to a certain extent, and the effect of the bacterial fertilizer was the most obvious. The application of 2% , The quality of fresh samples increased by 71.9% and 71.6% respectively, and increased by 155.9% and 153.1% in September. The effect of applying 2% of bacterium manure in September was more obvious than that of bacterial bran. The amount of soil microbes increased with the application of 2% fungus bran and ND35, and the number of soil bacteria and actinomycetes increased with the increase of treatment time, the highest in September. The amount of bacteria increased by 245.9%, 89.1% and 216.3% respectively compared with the control in July, August and September, and the numbers of actinomycetes increased by 68.7%, 124.7% and 131.1% respectively compared with the control. Soil enzyme activities were increased by both treatments. Soil urease, sucrase and phosphatase activities were the highest in August. The most obvious increase was soil enzyme activity with 2% bacterial fertilizer application. The application of 2% bacterial fertilizer also increased the root activity of the Pingma sweet tea plants, increasing by 59.0%, 50.2% and 20.2% respectively over the control in July, August and September. Compared with the control and the application of bran bran, the ND35 microbial fertilizer could better improve the microflora, improve soil enzyme activity and increase the root activity and biomass of the seedlings of Malus hupehensis.