目的观察消痰散结方对MKN-45人胃癌裸鼠原位移植瘤微卫星不稳定的抑制作用。方法采用MKN-45人胃癌细胞株建立裸鼠皮下瘤模型皮下传3代作为实验模型的瘤源,采用OB胶粘合法建立人胃癌裸鼠原位移植瘤模型。30只模型裸鼠分为模型组、中药组和化疗组,每组10只。中药组给予消痰散结方(0.4mL/天)灌胃,化疗组给予喃氟啶0.4mL/天灌胃,模型组无干预措施。用药6周后,测瘤质量、计算抑瘤率,检测5个微卫星不稳定位点(D17S250、D2S123、D5S346、Bat-25和BAT26)的大小、峰高度及峰面积。结果中药组抑瘤率为40.84%,中药组的瘤质量明显低于模型组(P<0.01),与化疗组比较,差异无统计学意义(P>0.05)。模型组高度微卫显不稳定发生率为70%,低度微卫星不稳定发生率为30%,使用消痰散结方治疗6周后,微卫星位点趋于稳定,均表现为微卫星稳定。结论消痰散结方对微卫星不稳定MKN-45人胃癌裸鼠原位移植瘤有抑制作用。 Objective To observe the inhibitory effect of Xiaotansanjie recipe on microsatellite instability of orthotopically transplanted MKN-45 human gastric cancer in nude mice. Methods The MKN-45 human gastric cancer cell line was used to establish the subcutaneous tumor-bearing model of nude mice and the third generation was used as the tumor source. The in situ xenograft model of human gastric cancer was established by OB glue method. 30 nude mice were divided into model group, Chinese medicine group and chemotherapy group, with 10 rats in each group. The traditional Chinese medicine group was given Xiaotan Sanjie prescription (0.4mL / day) by intragastric administration, and the chemotherapy group was treated with 0.4ml / day of flumine 0.4g / day. There was no intervention in the model group. After 6 weeks of treatment, tumor mass was measured and tumor inhibition rate was calculated. The size, peak height and peak area of ​​5 microsatellite instability sites (D17S250, D2S123, D5S346, Bat-25 and BAT26) were detected. Results The tumor inhibition rate of TCM group was 40.84%. The tumor mass of TCM group was significantly lower than that of model group (P <0.01). There was no significant difference between chemotherapy group and chemotherapy group (P> 0.05). The incidence of microsatellite instability in the model group was 70%, and the incidence of microsatellite instability was 30%. After 6 weeks of treatment with Xiaotan Sanjie Decoction, the microsatellite loci tended to be stable, both of which showed microsatellite stable. Conclusion Xiaotansanjie prescription has inhibitory effects on the xenografts in nude mice bearing microsatellite instable MKN-45 human gastric cancer.