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目的研究程氏蠲痹汤加减方对佐剂性关节炎(AA)大鼠滑膜T细胞受体前列腺素E受体4(PTGER4)水平的影响。方法通过大鼠足跖部皮下注射Freund完全佐剂配合冰水浴和吹风制作AA模型,检测各组大鼠关节肿胀度和关节炎指数,实时荧光定量PCR检测程氏蠲痹汤加减方对滑膜组织PTGER4 mRNA水平的影响。结果程氏蠲痹汤加减方能显著减轻AA模型大鼠关节、滑膜组织的破坏;高剂量(生药含量4 g/mL)处理的大鼠滑膜样本中PTGER4 mRNA水平明显降低。结论程氏蠲痹汤加减方能够降低大鼠AA滑膜组织PTGER4 mRNA水平。
Objective To study the effect of Chengshi Huibi Decoction on the level of T cell receptor PTGER4 in synovial membrane of adjuvant arthritis (AA) rats. Methods AA model was established by subcutaneous injection of Freund’s complete adjuvant with ice-water bath and hair blowing in the foot of the rats’ feet. The degree of joint swelling and arthritis index were detected in each group. Real-time fluorescent quantitative PCR was used to detect the effect of Chengshi Huibi Decoction on synovium Effect of tissue PTGER4 mRNA levels. Results Cheng’s Yi Bi Decoction can significantly reduce the AA model rats joint and synovial tissue damage; high-dose (crude drug content 4 g / mL) treated rat synovial samples PTGER4 mRNA levels were significantly lower. Conclusion Chengbianbibi Decoction can reduce the level of PTGER4 mRNA in rat AA synovial tissue.