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目的探讨维生素Ｅ对体外培养的人眼小梁细胞糖胺多糖（ｇｌｙｃｏｓａｍｉｎｏｇｌｙｃａｎ，ＧＡＧ）代谢的影响。方法第３～５代人眼小梁中加入１００、２００、４００、８００μｇ／ｍｌ的ＶｉｔＥ，分离提纯后ＧＡＧ经腊酸纤维薄膜电泳、闪烁测量测得人眼小梁细胞ＧＡＧ的总量和各组份构成比。结果１００、２００、４００μｇ／ｍｌＶｉｔＥ能增加ＧＡＧ的总量，同时增加透明质酸的构成比，降低含硫ＧＡＧ的构成比。８００μｇ／ｍｌＶｉｔＥ降低ＧＡＧ总量而不改变其构成比。结论ＶｉｔＥ似有在发病学上防治ＰＯＡＧ的可能性。
Objective To investigate the effect of vitamin E on the metabolism of glycosaminoglycan (GAG) in cultured human trabecular meshwork cells. Methods 100, 200, 400 and 800μg / ml VitE were added into the trabecular meshwork of the third to fifth generation of the method. After separation and purification, the GAG was electrophoresed on the sialic acid fiber membrane and the total amount of GAG in human eye trabecular meshwork Composition ratio. Results 100, 200, 400μg / ml VitE can increase the total amount of GAG, while increasing the composition ratio of hyaluronic acid, reducing the composition ratio of sulfur-containing GAG. VigE at 800 μg / ml reduced the total amount of GAG without changing its constituent ratio. Conclusion VitE seems to have the potential to prevent and treat POAG.