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应用寡聚核苷酸基因芯片,分析了米亚罗林区冷杉原始林(M-Y)和20世纪60年代云杉人工林(M-60)土壤微生物的功能基因多样性。该功能基因芯片含有与有机碳降解、碳固定、氮、磷、硫循环和金属抗性相关的1961个基因探针。在M-Y和M-60样地中分别检测到39和62个具有较强杂交信号(SNR≥2)的功能基因,其基因多样性水平指数分别为3.59和4.04,杂交信号强度总值分别为480280和630560。M-Y和M-60样地中分别检测到32个和37个有机碳降解基因,占总基因的82%和60%,这些基因分属于22个不同的基因类群,分别参与木质素、木聚糖、几丁质等有机碳的降解过程。有机碳降解基因在两个样地中存在较大的多样性和丰度差异。这些结果说明了大多数的土壤微生物直接参与了土壤有机碳的降解,同时,林型不同显著影响了土壤微生物群落结构和有机碳降解微生物的多样性。
By using oligonucleotide microarray, the functional gene diversity of soil microorganisms in Miaolian fir native forest (M-Y) and 1960s spruce plantation (M-60) was analyzed. The functional gene chip contains 1961 probe probes related to organic carbon degradation, carbon fixation, nitrogen, phosphorus, sulfur cycling and metal resistance. In MY and M-60 plots, 39 and 62 functional genes with strong hybridization signal (SNR≥2) were detected respectively, and their gene diversity index were 3.59 and 4.04 respectively, and the total signal intensity of hybridization was 480280 And 630560. 32 and 37 organic carbon-degrading genes were detected in MY and M-60 plots, accounting for 82% and 60% of the total, respectively. These genes belonged to 22 different gene groups, which were involved in lignin, xylan , Chitin and other organic carbon degradation process. Organic carbon degradation genes in the two plots there is a greater diversity and abundance differences. These results indicate that most of the soil microorganisms are directly involved in the degradation of soil organic carbon, and at the same time, the different forest types significantly affect the microbial community structure and the diversity of organic carbon-degrading microorganisms.