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目的:探讨创伤应激时大鼠胃动力的变化以及胃肠动力调节相关神经肽血管活性肠肽(VIP)及胆囊收缩素-8(CCK-8) 对它的影响。方法:于大鼠胃窦部缝置应力传感器,将大鼠双侧后肢骨膝关节以上钳断,制备应激模型。记录应激前后大鼠胃动力的变化及血浆内VIP和CCK-8 含量的改变,观察应激前后静脉插管内给予VIP和CCK-8 后胃动力变化。结果:正常大鼠禁食状态,胃运动呈规则的相对静止期、收缩期交替的变化。VIP可抑制正常大鼠胃运动,CCK-8明显加强胃的收缩。应激使大鼠胃窦的规则收缩消失、运动加强;大鼠血浆内VIP含量在应激后1,2 h 显著升高, CCK-8 含量于应激后即刻升高,随后恢复正常。相同剂量的VIP对增强的胃运动有明显的抑制效应。结论:创伤应激可致大鼠胃动力紊乱及体内胃肠动力调节相关神经肽VIP,CCK-8分泌改变,VIP对增强的胃动力有抑制作用。
Objective: To investigate the effects of traumatic stress on gastric motility and the effects of gastrointestinal motility regulating neuropeptide VIP and CCK-8 in rats. METHODS: Stress sensors were placed in the antrum of the rat and the buttocks and joints of the bilateral hindlimbs were clamped above each other to prepare a stress model. Changes of gastric motility and content of VIP and CCK-8 in plasma were recorded before and after stress, and the changes of gastric motility after VIP and CCK-8 administration were observed before and after venous catheterization. Results: The fasting state of normal rats, the relative stationary phase of gastric motility and the alternation of systolic phase. VIP can inhibit normal rat gastric motility, CCK-8 significantly enhance gastric contraction. The regular contraction of gastric antrum disappeared and the exercise was strengthened by stress. The content of VIP in plasma of rats significantly increased at 1 and 2 h after stress, and the content of CCK-8 increased immediately after stress and then returned to normal. The same dose of VIP has a significant inhibitory effect on enhanced gastric motility. CONCLUSION: Traumatic stress can cause disturbance of gastric motility in rats and secretion of VIP, CCK-8 related to gastrointestinal motility regulation in vivo. VIP has inhibitory effect on enhanced gastric motility.