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目的探讨松弛素受体1(RXFP1)在石英尘致矽肺中的潜在作用。方法无特定病原体级雄性Wistar大鼠64只随机分为对照组和染尘组。采用非暴露式气管内一次性灌注法,染尘组予气管内灌入质量浓度为500 g/L德国标准石英混悬液0.1 m L,对照组予气管内灌入0.9%氯化钠溶液0.1 m L。2组大鼠分别在染毒第1、7、14和28天各处死8只,以苏木精-伊红染色观察大鼠肺组织的病理学变化,分别用实时荧光定量聚合酶链反应法和免疫组织化学法分析大鼠肺组织中Rxfp1 mRNA和RXFP1蛋白的表达。结果染尘第28天,染尘组大鼠双肺肉眼可见灰白色结节,光镜下可观察到肺泡间隔断裂,局部可见明显的矽结节。染尘组大鼠肺组织Rxfp1 mRNA相对表达水平于染尘第1天升高至对照组的145%(P<0.01),于第7和14天下降至与对照组接近水平(P>0.05),于第28天下降至对照组的45%(P<0.01)。染尘组大鼠肺组织中RXFP1蛋白相对表达水平从染毒第7天开始高于对照组(P<0.01),第28天达到最高水平(P<0.01)。结论 RXFP1可能在抑制矽肺发病过程中发挥重要作用。
Objective To investigate the potential role of RXFP1 in quartz-induced silicosis. Methods Sixty-four male Wistar rats without specific pathogen were randomly divided into control group and dyed group. The non-exposed intra-tracheal instillation method was used. In the dust-exposed group, intratracheal instillation of German standard quartz suspension with a concentration of 500 g / L was performed at a concentration of 0.1 mL. The control group was infused with 0.9% sodium chloride solution m L. Two groups of rats were sacrificed on the 1st, 7th, 14th and 28th day of exposure respectively, and the pathological changes of the lung tissue were observed by hematoxylin-eosin staining. Real-time fluorescent quantitative polymerase chain reaction Immunohistochemistry was used to analyze the expression of Rxfp1 mRNA and RXFP1 protein in rat lung tissue. Results On the 28th day of dying, gray nodules were visible in both eyes of the rats in the dyed dust group. Alveolar septal fracture was observed under the light microscope, and the obvious silicon nodules were visible locally. The relative expression level of Rxfp1 mRNA in the lung tissue of the dust-exposed group increased to 145% (P <0.01) of the control group on the 1st day after exposure to dust, and decreased to the level of the control group on the 7th and 14th days (P> 0.05) , Dropped to 45% of the control group on day 28 (P <0.01). The relative expression level of RXFP1 in the lung tissue of the dust-exposed group was higher than that of the control group on the 7th day (P <0.01), and reached the highest level on the 28th day (P <0.01). Conclusion RXFP1 may play an important role in inhibiting the pathogenesis of silicosis.