论文部分内容阅读
为研究内皮素 1(ET 1)对培养的人肺动脉平滑肌细胞 (HPASMC )I、Ⅲ型胶原合成的影响 ,应用贴块法培养人胎儿肺动脉平滑肌细胞 ,应用免疫细胞化学染色、原位杂交方法检测I、Ⅲ型胶原蛋白及其mRNA的表达。结果显示 ,加入含 10 - 8mol LET 1的培养液培养 4 8h和 72h后 ,HPASMCI、Ⅲ型前胶原mRNA及其蛋白表达明显增强。提示ET 1能够诱导培养的人肺动脉平滑肌细胞I、Ⅲ型胶原合成增加 ,其调控机制之一是在转录水平上增强了I、Ⅲ型前胶原mRNA的表达
In order to study the effect of endothelin 1 (ET 1) on the synthesis of type I and type III collagen in cultured human pulmonary artery smooth muscle cells (HPASMC), human fetal pulmonary artery smooth muscle cells were cultured by patch method and detected by immunocytochemistry and in situ hybridization Type I and type Ⅲ collagen and its mRNA expression. The results showed that the expression of mRNA and protein of HPASMCI and procollagen type Ⅲ increased significantly after 48 h and 72 h culture with 10 - 8 mol LET 1. These results suggest that ET 1 can induce the synthesis of type I and type III collagen in cultured human pulmonary artery smooth muscle cells. One of the regulatory mechanisms is that the expression of type I and type III procollagen mRNA is enhanced at the transcriptional level