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目的 探讨 P16基因的突变在人胰腺癌发生及侵袭转移中的作用 ,为临床提供有益的帮助。方法 应用 PCR、PCR-SSCP、DNA测序技术 ,研究 P16基因的突变情况。结果 在 35患者胰腺癌标本中 ,发现有 12例在 P16基因第二外显子部分存在至少 5 2 2 bp的纯合缺失、另有 7例存在 2个点突变 ,总突变率为 5 4.3% ,而癌旁组织无 P16基因的突变 ,统计学分析表明 P16基因的突变与人胰腺癌多种生物学行为及临床分期 期有明确的关系。结论 研究结果提示 P16基因的突变人胰腺癌的发生、组织分化、浸润程度、淋巴结转移及临床分期有明显的相关性 ,有助于判断胰腺癌的恶性程度和患者的预后。
Objective To investigate the role of P16 gene mutation in the genesis and invasion and metastasis of human pancreatic cancer and provide useful clinical help. Methods The mutation of P16 gene was studied by PCR, PCR-SSCP and DNA sequencing. Results In 35 pancreatic cancer samples, there were 12 cases with at least 522 bp homozygous deletion in the second exon of P16 gene, and another 7 cases had 2 point mutations with a total mutation rate of 53.3% , While the adjacent tissue without P16 gene mutation, statistical analysis showed that P16 gene mutation and human pancreatic cancer a variety of biological behavior and clinical stage has a clear relationship. Conclusion The results suggest that P16 gene mutation in human pancreatic cancer, histological differentiation, infiltration, lymph node metastasis and clinical stage have a clear correlation, which can help to determine the degree of pancreatic cancer and prognosis.