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利用脱氧肾上腺素抗体与变肾上腺素强交叉反应建立起灵敏、简单的血浆变肾上腺素放射免疫测定法。经方法考验,标准曲线的精密度为3.7±1.4%,最小检出量小于2pg/管;批内、批间误差分别为3.8~6.3%和7.6~11.6%,平均回收率为90%左右;加入不同量的血浆与测得变肾上腺素具有线性关系。正常人卧位血浆变肾上腺素浓度为65.6±56.4(13~147)pg/ml。血浆与尿中变肾上腺素呈显著正相关r=0.747(n=97 p<0.01游离);r=0.747(n=97 p<0.01总)。速尿和冰水冷应激未使变肾上腺素明显升高,但狗在麻醉状态下人为增加颅内压后变肾上腺素明显变化,血浆变肾上腺素可作为交感神经活性和嗜铬细胞瘤诊断的指标。
A sensitive and simple plasma adrenergic radioimmunoassay was established using a strong cross-reaction between the adrenergic antibody and adrenergic. According to the method test, the precision of the standard curve was 3.7 ± 1.4% and the minimum detectable amount was less than 2pg / tube. The error between the batch and the batch was 3.8-6.3% and 7.6-11.6%, respectively. The average recovery was about 90% Adding a different amount of plasma measured with adrenaline has a linear relationship. Normal human supine plasma adrenaline concentrations of 65.6 ± 56.4 (13 ~ 147) pg / ml. Plasma and urinary adrenaline levels were significantly and positively correlated with r = 0.747 (n = 97 p <0.01 free); r = 0.747 (n = 97 p <0.01 total). Rapid urease and ice-cold stress did not make the adrenergic significantly increased, but the dogs under anesthesia artificially increased after intracranial pressure changes significantly adrenergic, plasma adrenergic can be used as a sympathetic nerve and pheochromocytoma diagnosis index.