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建立了一种核壳型SiO2荧光纳米粒子标记的激光诱导荧光免疫测定的新方法.该方法用Ru(bpy)32+掺杂的核壳型SiO2荧光纳米粒子为标记物,采用双抗体夹心法,在载玻片上固定鼠抗人癌胚抗原(CEA)单克隆抗体,加入1～2μL样品,再加入纳米粒子标记的另一种鼠抗人癌胚抗原(CEA)单克隆抗体.识别后在光导纤维激光诱导荧光毫米阵列检测平台上测量荧光信号,进行定量.在优化条件下,荧光强度和癌胚抗原的量在1～80 pg的范围内具有良好的线性关系,相关系数r=0.9933,方法的检出限为0.3 pg(20 amol).对40 pg的CEA平行测定5次,相对标准偏差为5.5%.
A new method of laser-induced fluorescence immunoassay for the detection of core-shell SiO2 fluorescent nanoparticles was established.The core-shell SiO2 fluorescent nanoparticles doped with Ru (bpy) 32+ were used as labels and the double antibody sandwich method , Immobilize mouse anti-human carcinoembryonic antigen (CEA) monoclonal antibody on glass slides, add 1 ~ 2μL sample, then add another mouse anti-human carcinoembryonic antigen (CEA) monoclonal antibody labeled by nano-particle.After identification, Under optimized conditions, the fluorescence intensity and the amount of carcinoembryonic antigen have a good linear relationship in the range of 1 ~ 80 pg, the correlation coefficient r = 0.9933, The limit of detection was 0.3 pg (20 amol) and the relative standard deviation (RSD) was 5.5% with 40 pg of CEA measured in parallel.