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目的:探讨胰岛素样生长因子-1(IGF-1)在卵巢上皮性肿瘤组织中的表达,观察IGF-1反义寡核苷酸对卵巢癌细胞系3AO凋亡及侵袭能力的影响。方法:免疫组织化学方法检测60例卵巢上皮性癌、20例卵巢上皮性交界性肿瘤、20例卵巢良性肿瘤及20例正常卵巢组织中IGF-1蛋白表达;将3AO分为4组,用脂质体介导方法将IGF-1反义寡核苷酸转入其中,流式细胞仪AnnexinⅤ/PI双染色法检测其凋亡,Boyden小室检测其侵袭能力。结果:IGF-1在卵巢上皮性癌组、交界性组、良性组及正常卵巢组的阳性表达率分别为61.6%、25.0%、15.0%和10.0%,上皮癌组与其他3组比较差异有统计学意义,P<0.05;后3组之间比较差异无统计学意义,P>0.05。临床期别晚组及组织分化低组IGF-1阳性表达率为67.4%和81.8%,高于临床期别早组及组织分化高组的35.7%和52.6%,差异有统计学意义,P<0.05。IGF-1的阳性表达与发病年龄、有无腹水无关,≥50岁组阳性表达率为59.8%,<50岁组阳性表达率为54.0%,P>0.05;有腹水组阳性表达率为66.0%,无腹水组阳性表达率为52.0%,P>0.05。IGF-1反义寡核苷酸组卵巢癌细胞的凋亡率为(45.5±0.1)%,与对照组的(16.5±0.4)%比较差异有统计学意义,P<0.05;IGF-1反义寡核苷酸组癌细胞穿透Matri-gel胶的细胞数目为(42±6)个,与对照组的(132±26)个比较差异有统计学意义,P<0.05。结论:IGF-1表达与卵巢上皮性肿瘤的分期、分化有关,抑制IGF-1的表达能增加卵巢癌细胞凋亡,降低其侵袭能力。
Objective: To investigate the expression of insulin-like growth factor-1 (IGF-1) in epithelial ovarian cancer and to observe the effect of IGF-1 antisense oligonucleotide on the apoptosis and invasion of ovarian cancer cell line 3AO. Methods: The expression of IGF-1 in 60 cases of epithelial ovarian cancer, 20 cases of ovarian epithelial borderline tumor, 20 cases of benign ovarian tumor and 20 cases of normal ovarian tissue were detected by immunohistochemistry. 3AO was divided into 4 groups, The antisense oligonucleotides of IGF-1 were transfected into them by plastid-mediated method. Apoptosis was detected by flow cytometry Annexin Ⅴ / PI double staining and Boyden chamber was used to detect the invasion ability. Results: The positive rates of IGF-1 in epithelial ovarian cancer group, borderline group, benign group and normal ovary group were 61.6%, 25.0%, 15.0% and 10.0% respectively. The difference between the two groups was significant Statistical significance, P <0.05; There was no significant difference in the latter three groups (P> 0.05). The positive expression rate of IGF-1 in the late clinical group and low differentiation group was 67.4% and 81.8%, which was higher than that in early clinical group and high differentiation group (35.7% and 52.6%, P < 0.05. The positive expression of IGF-1 was related to the age of onset and the presence or absence of ascites. The positive expression rate of IGF-1 was 59.8% in the group of ≥50 years, the positive rate was 54.0% in the group of <50 years old, P> 0.05; , No ascites group positive expression rate was 52.0%, P> 0.05. The apoptosis rate of ovarian cancer cells in IGF-1 antisense oligonucleotide group was (45.5 ± 0.1)%, which was significantly different from that in control group (16.5 ± 0.4)%, P <0.05; IGF- The number of cells penetrating Matri-gel in the oligonucleotide group was (42 ± 6), which was significantly lower than that in the control group (132 ± 26), P <0.05. Conclusion: The expression of IGF-1 is related to the staging and differentiation of epithelial ovarian tumor. Inhibition of IGF-1 expression can increase the apoptosis of ovarian cancer cells and decrease their invasiveness.