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以虎皮香菇（Ｌｅｎｔｉｎｕｓｔｉｇｒｉｎｕｓ）为材料，以酶解法分离原生质体，进行培养，获得再生菌丝。本研究主要结论是：（１）适合于分离原生质体的菌丝菌龄以２４～４８ｈ为佳，以１００目筛过滤得到的菌丝片段进行继代培养较不处理的菌丝体为好；（２）酶解温度确定在３３℃左右情况下，采用１％Ｌｙｗａｌｌｚｙｍｅ和１％Ｃｅｌｌｕｌａｓｅ，以０．６５ｍｏｌ／ＬＭｇＳＯ４为稳渗剂，酶解４ｈ，采用４００目不锈钢网除去未被酶解的菌丝片段；每克湿菌丝的原生质体产量可达４．５×１０７个／ｍＬ；（３）原生质体再生是一个间歇的过程。
Lentinus tigrinus was used as raw material to isolate protoplasts by enzymolysis and culture to obtain regenerated mycelium. The main conclusions of this study are as follows: (1) The suitable mycelial age of the mycelia isolated from protoplasts is 24 ~ 48h, mycelial fragments obtained by 100 mesh sieve filtration are better than the untreated mycelia. (2) The hydrolysis temperature was determined at about 33 ℃, the use of 1% Lywallzyme and 1% Cellulase, with 0.65mol / LMgSO4 as stabilizing agent, hydrolysis 4h, 400 mesh stainless steel mesh was removed without enzymatic hydrolysis Silk fragments; per gram of wet mycelium protoplast yield up to 4.5 × 107 / mL; (3) protoplast regeneration is a batch process.