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目的探讨呆聪液对痴呆大鼠模型脑内β-淀粉样前体蛋白(β-APP)基因表达的影响。方法22月龄SD大鼠采用海人酸破坏脑基底核法造成学习和记忆功能障碍的痴呆动物模型。造模后的大鼠随机分为痴呆模型组、呆聪液(低、中、高剂量)组,同时设正常对照组,每组10只。呆聪液低、中、高剂量组每日呆聪液灌胃(低5g/kg、中10g/kg、高20g/kg),痴呆模型组和正常对照组每天用生理盐水(10ml/kg)灌胃,共1个月。通过水迷宫法和反转录聚合酶链反应观察呆聪液对痴呆模型大鼠学习记忆能力和脑内β-APP基因表达的影响。结果老龄痴呆模型大鼠学习记忆能力下降,脑内β-APP基因表达增强。呆聪液高、中、低剂量组能明显提高学习记忆能力,降低脑内β-APPmRNA含量,并有一定的量效关系,与痴呆模型组比较差异有显著性(P<0.05)。结论呆聪液能改善老龄痴呆大鼠的学习和记忆功能,下调β-APP基因的表达。
Objective To investigate the effect of Daicong Solution on the expression of β-amyloid precursor protein (β-APP) gene in brain of dementia rat model. Methods A 22-month-old SD rat model of dementia with kainic acid disrupting brain basal ganglia caused learning and memory dysfunction. After modeling, the rats were randomly divided into dementia model group and Daicong solution group (low, middle and high dose), and normal control group with 10 rats in each group. Daicong low-, medium-, and high-dose groups were given daily (at 5g/kg, medium 10g/kg, and high 20g/kg), and normal saline (10ml/kg) was used daily in the dementia model group and normal control group. Gavage, a total of 1 month. The effects of Daicong solution on learning and memory ability and expression of β-APP gene in brain of dementia model rats were observed by water maze and reverse transcription polymerase chain reaction. Results The learning and memory abilities of aged dementia model rats decreased, and the expression of β-APP gene in brain increased. The high-dose, medium-dose, and low-dose Dakcong groups significantly increased the ability of learning and memory, decreased the content of β-APPmRNA in the brain, and had a certain dose-effect relationship, and there was a significant difference compared with the dementia model group (P<0.05). Conclusion Daicong Solution can improve the learning and memory function of aged dementia rats and down-regulate the expression of β-APP gene.