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葡萄糖氧化酶催化葡萄糖被水中的溶解氧所氧化,生成葡萄糖酸和过氧化氢。生成的过氧化氢再在过氧化物酶的存在下将原来无色的邻联二茴香胺氧化成有色产物的反应是:
Glucose oxidase catalyzes the oxidation of glucose by dissolved oxygen in water, producing gluconic acid and hydrogen peroxide. The reaction of the resulting hydrogen peroxide to oxidize the otherwise colorless o-dianisidine to a colored product in the presence of peroxidase is: