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以小麦济南17和山农8355为材料,在低温胁迫条件下,测定了不同茎蘖功能叶和叶鞘超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MDA)和可溶性蛋白含量,并利用主成分分析、聚类分析对其抗冻性进行综合评价.结果表明:低温胁迫条件下,小麦拔节期不同茎蘖功能叶和叶鞘中SOD、POD和CAT活性均不同程度地上升,MDA和可溶性蛋白含量则不同程度地上升或下降.利用主成分分析和聚类分析,将济南17不同茎蘖分为3类:主茎和一级分蘖Ⅰ、Ⅱ属强抗冻蘖组,一级分蘖Ⅲ、Ⅳ和二级分蘖Ⅰp属中度抗冻蘖组,二级分蘖Ⅱp属弱抗冻蘖组;将山农8355不同茎蘖分为3类:主茎和一级分蘖Ⅰ、Ⅱ、Ⅲ属强抗冻蘖组,一级分蘖Ⅳ和二级分蘖Ⅰp属中度抗冻蘖组,二级分蘖Ⅱp属弱抗冻蘖组.表明冬小麦拔节期不同茎蘖存在抗冻性差异,且低位蘖较高位蘖抗冻.
Using wheat Jinan 17 and Shannong 8355 as materials, the contents of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and catalase (CAT) Activity and malondialdehyde (MDA) and soluble protein content, and using the principal component analysis and cluster analysis to evaluate the frost resistance.The results showed that under low temperature stress, The activities of SOD, POD and CAT all increased to different extents, and MDA and soluble protein contents increased or decreased to different extents.Using principal component analysis and cluster analysis, the different stem tillers of Jinan17 were divided into three groups: Tillers Ⅰ and Ⅱ were strongly anti-freezing tillering group, the first tillering Ⅲ and Ⅳ and the second tillering Ⅰp were moderate anti-freezing tillering group, the second tillering Ⅱp was weak and anti-freezing tillering group; the shanong 8355 different tillering was divided into Three types: the main stem and the first tillering Ⅰ, Ⅱ, Ⅲ is a strong anti-freezing tillering group, the first tillering Ⅳ and the second tillering Ⅰp moderate anti-freezing tillering group, the second tillering Ⅱp is weak anti-freezing tillering group. There were differences in frost resistance of different tillers at the jointing stage of winter wheat, and high tiller at low tillers was antifreeze.