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目的观察外周血基因组DNA端粒长度与肺癌危险性的关系。方法用荧光定量PCR法检测145例肺癌患者、145名正常对照和145名焦炉工人外周血基因组DNA相对端粒长度。结果肺癌组端粒长度明显短于对照组(P=0.028),职业暴露组端粒长度明显短于肺癌组(P=0.005)和对照组(P<0.001);按对照组端粒长度分4组,随着端粒的缩短,肺癌的危险性增加(Ptrend<0.001);按对照组端粒长度中位数把样本分为2组发现,与长端粒组比较,端粒变短明显增加肺癌的危险性(调整OR=2.337,95% CI为1.413～3.866);对照组中,随着年龄增加,端粒缩短(P<0.001)。结论端粒缩短可增加肺癌的危险性,可能是肺癌发展早期过程中的一项重要的生物标志。
Objective To observe the relationship between the telomere length of peripheral blood genomic DNA and the risk of lung cancer. Methods The relative telomere length of genomic DNA in peripheral blood of 145 patients with lung cancer, 145 healthy controls and 145 coke oven workers was detected by real-time fluorescence quantitative PCR. Results The length of telomere in lung cancer group was significantly shorter than that in control group (P = 0.028). The length of telomere in occupational exposure group was significantly shorter than that in lung cancer group (P = 0.005) and control group (P <0.001) Group, with the telomere shortening, the risk of lung cancer increased (Ptrend <0.001). According to the median telomere length in the control group, the samples were divided into two groups. Compared with the long telomere group, the telomere shortening was significantly increased The risk of lung cancer (adjusted OR = 2.337, 95% CI 1.413 ~ 3.866); in the control group, telomere shortened with age (P <0.001). Conclusion Telomere shortening may increase the risk of lung cancer and may be an important biomarker in the early stage of lung cancer.