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研究氟西汀对小鼠恐惧记忆形成过程中海马、前额叶皮质及杏仁核BDNF和Bcl-2表达的影响。取48只雄性ICR小鼠,随机分为空白对照组、条件性恐惧刺激组(conditioned fear group,CF)和氟西汀+条件性恐惧刺激组(FLX+CF group)。电击刺激后1 h和24 h分别观察僵立时间;Western blotting检测海马、前额叶皮质及杏仁核BDNF、Bcl-2的表达。结果显示,24 h后,FLX+CF组僵立时间百分比为(19.4±2.2)%,显著低于CF组(29.9±4.2)%;电击刺激1天后,FLX+CF组海马Bcl-2蛋白表达显著增加(P<0.001),BDNF表达无明显差异;7天后,Bcl-2蛋白表达无明显差异,BDNF表达显著增加(P<0.001);额叶及杏仁核BDNF和Bcl-2表达与CF组相比无明显差异。结果表明,氟西汀通过上调海马Bcl-2及BDNF的表达,发挥中枢神经保护作用,从而影响恐惧记忆的形成。
To study the effect of fluoxetine on the expression of BDNF and Bcl-2 in hippocampus, prefrontal cortex and amygdala during the formation of fear memory in mice. Forty eight male ICR mice were randomly divided into blank control group, conditioned fear group (CF) and fluoxetine plus conditioned fear stimulation group (FLX + CF group). The freezing time was observed at 1 h and 24 h after electrical shock stimulation respectively. The expressions of BDNF and Bcl-2 in hippocampus, prefrontal cortex and amygdala were detected by Western blotting. The results showed that the percentage of dead time in FLX + CF group was (19.4 ± 2.2)% after 24 h, which was significantly lower than that in CF group (29.9 ± 4.2)%. Bcl-2 protein expression in FLX + CF group (P <0.001). There was no significant difference in BDNF expression between the two groups (P <0.001). After 7 days, there was no significant difference in Bcl-2 protein expression but BDNF expression (P <0.001). BDNF and Bcl- No significant difference compared to. The results show that fluoxetine can play a central nervous protective role by up-regulating the expression of Bcl-2 and BDNF in the hippocampus, thus affecting the formation of fear memory.