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目的:建立简单快速的枸杞总黄酮测定方法,同时对其水煎煮的提取动力学过程进行模拟,建立动力学方程并验证。方法:采用紫外分光光度法,对其方法学进行考察;使用该方法测定不同提取时间和溶剂倍量下枸杞水提液中总黄酮浓度,以简化的数学动力学模型为基础,推算出方程参数进行方程拟合。结果:芦丁在0.004 828~0.028 968 g·L-1具有良好的线性关系,回归方程为Y=29.823x+0.015 8,r=0.999 6,平均回收率为99.93%,RSD=0.76%;拟合的动力学模型结果为:CB=(4.874 8×t0.5/M-1.018 3)0.666 256,验证结果良好。结论:所简化的枸杞总黄酮测定方法稳定可行,重现性好。模型适用并能良好的描述枸杞水提取的动力学过程。
OBJECTIVE: To establish a simple and rapid method for the determination of total flavonoids in Lycium barbarum L. Simultaneously, the extraction kinetic process of water decoction was simulated and the kinetic equation was established and verified. Methods: The method of UV spectrophotometry was used to investigate its methodology. The method was used to determine the total flavonoids concentration of Lycium barbarum aqueous extract under different extraction time and solvent times. Based on the simplified mathematical model, the equation parameters Fit the equation. Results: The calibration curve of rutin was linear in the range of 0.004 828 to 0.028 968 g · L-1. The regression equation was Y = 29.823x + 0.015 8, r = 0.999 6, with an average recovery of 99.93% and RSD of 0.76% The results of the kinetic model are as follows: CB = (4.874 8 × t0.5 / M-1.018 3) 0.666 256, the result of validation is good. Conclusion: The simplified method for the determination of total flavonoids in Lycium barbarum is stable and reproducible. The model is suitable and can well describe the kinetic process of wolfberry water extraction.