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人体IgA两个亚类的初级结构、碳水化合物的组成、抗原决定簇与对微生物的蛋白分解酶的敏感性是不同的.IgA_1和IgA_2在体液中的分布也有所不同.80%或以上的血清IgA属IgA_1,外分泌液中IgA_1占总IgA的50-74%.IgA_2在外分泌液中相对增多的原因不明,可能是由于远端组织产生的IgA_2首先输入外分泌液之故,也可能与具有分泌表面的组织含有IgA_2细胞数增多有关.为了证实以上情况,作者用抗IgA_1和IgA_2的多克隆抗体及自制的抗人骨髓瘤IgA_1和IgA_2的小鼠单克隆抗体,用间接免疫荧光技术检测不同组织中的浆细胞.骨髓
The primary structure of the two subclasses of human IgA, the composition of the carbohydrate, the antigenic determinant, is different from the susceptibility of the microbial proteolytic enzyme, and the distribution of IgA_1 and IgA_2 in the bodily fluid is also different: 80% or more of serum IgA is IgA_1, exocrine IgA_1 accounted for 50-74% of total IgA IgA_2 in the relative increase in exocrine fluid for unknown reasons, probably due to the distal tissue produced by the first input of exocrine IgA_2, it may also have a secretory surface .In order to verify the above situation, the authors used the anti-IgA_1 and IgA_2 polyclonal antibodies and homemade anti-human myeloma IgA_1 and IgA_2 mouse monoclonal antibodies, indirect immunofluorescence assay in different tissues Plasma cells. Bone marrow