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目的 :利用微波免疫组化法检测膀胱癌 P5 3基因蛋白表达并分析其临床意义。方法 :通过微波辐射重新暴露福尔马林固定而掩敝的组织抗原 ,利用链霉亲合素生物素免疫组化染色法 ( L SAB)对 3 8例膀胱癌 P5 3蛋白表达进行研究 ,并结合随访 ,观察其预后。结果 :3 8例膀胱癌中 ,17例 P5 3蛋白表达阳性 ,阳性率为 44 .7% ,并随肿瘤病理分期、病理分级增高 ,阳性率逐渐增高。膀胱癌 P5 3蛋白阳性表达与阴性表达间存活时间有显著性差异 ( P<0 .0 1)。结论 :膀胱癌 P5 3蛋白表达与其恶性程度、生存期及预后密切相关
Objective: To detect the expression of P53 protein in bladder cancer by microwave immunohistochemistry and analyze its clinical significance. Methods: The fixed and occluded tissue antigens were re-exposed by microwave radiation. The expression of P53 protein in 38 cases of bladder cancer was studied by streptavidin biotin immunohistochemical staining (L SAB) Combined with follow-up, observe the prognosis. Results: Of the 38 cases of bladder cancer, 17 cases had positive expression of P53 protein, with a positive rate of 44.7%. The positive rate of P5 3 protein was also increased with the pathological stage and pathological grade. There was a significant difference in the survival time between P5 3 protein positive and negative expression in bladder cancer (P <0.01). Conclusion: The expression of P53 protein in bladder cancer is closely related to its malignancy, survival and prognosis