论文部分内容阅读
研究了外源油菜素内酯(BL)对NaCl胁迫下棉花幼苗的钠累积、叶片生理特征及叶片差异基因表达水平的影响.结果表明:NaCl胁迫下2个棉花品种各部位钠含量升高,叶片丙二醛(MDA)、脯氨酸含量上升,叶绿素含量下降,叶片基因表达水平受到影响.外源施用油菜素内酯可降低NaCl胁迫下棉花幼苗根、茎、叶的钠含量,降低叶片中MDA含量,提高脯氨酸含量,并且NaCl胁迫下苏棉12号棉花品种更易受油菜素内酯调控.对苏棉12号的叶片进行数字表达谱分析结果表明,NaCl胁迫下苏棉12号叶片中的差异基因表达水平受到油菜素内酯调控,BL+NaCl处理棉花叶片的基因表达模式与CK(正常生长棉株)更为一致.说明外源油菜素内酯可减轻NaCl胁迫下棉花叶片受抑制程度,从而使NaCl胁迫下棉花叶片生理功能增强(叶绿素含量升高),最终使生物量增加.
The effects of exogenous brassinosteroid (BL) on the sodium accumulation, physiological characteristics of leaves and the differential gene expression in leaves of cotton seedlings under NaCl stress were studied.The results showed that the sodium content of two cotton cultivars increased under NaCl stress, Leaf MDA content, proline content increased, chlorophyll content decreased, leaf gene expression levels are affected.Exogenous application of brassinosteroids can reduce the NaCl content in roots, stems and leaves of cotton seedlings, reduce leaf MDA content and proline content increased, and under the stress of NaCl stress, the cotton varieties of Su cotton 12 were more easily regulated by brassinolide.The digital analysis of the leaves of Sumian 12 showed that under the NaCl stress, The expression level of differentially expressed genes in leaves was regulated by brassinosteroid, and the gene expression pattern of cotton leaves treated by BL + NaCl was more consistent with that of CK (normal-grown cotton plants), indicating that exogenous brassinosteroids can reduce cotton leaves under NaCl stress Under the NaCl stress, the physiological function of cotton leaves increased (chlorophyll content increased), which ultimately increased the biomass.