用可见光光谱法快速测定工业发酵液中液体脂肪的含量。用无水乙醇作溶剂,异辛烷作萃取剂,醋酸铜作显色剂,以可见光光谱在680～700nm波长处测OD值,根据回归方程可计算脂肪油含量。结果表明,液体脂肪浓度在0.2～2.0mg/mL之间呈良好线性关系(r=0.9992),平均回收率为107.09%,RSD为1.34%(n=5)。实验证明,采用此方法比其他方法简便、准确、重现性好。 Rapid Determination of Liquid Fat in Industrial Fermentation Broth by Visible Light Spectroscopy. Using absolute ethanol as solvent, isooctane as extractant and copper acetate as chromogenic agent, the OD value was measured by visible spectrum at the wavelength of 680-700 nm, and the fatty oil content could be calculated according to the regression equation. The results showed that there was a good linear relationship between the concentration of liquid fat and 0.2 ~ 2.0mg / mL (r = 0.9992), the average recovery was 107.09% and the RSD was 1.34% (n = 5). Experiments show that this method is more convenient, accurate and reproducible than other methods.