本文报道以荧光原位杂交技术（ＦＩＳＨ）及抗着丝粒抗体间接免疫荧光染色（ＣＲＥＳＴ染色）两种技术检测了化合物甲基丙烯酸环氧丙酯（ＧＭＡ）诱发微核的着丝粒信号比例，从而判断ＧＭＡ的非整倍体诱发效应。结果表明，ＧＭＡ诱发的微核约４０．９－５７５％为ＦＩＳＨ阳性，说明其具有一定的非整倍体毒性，但ＣＲＥＳＴ阳性仅为１７０－２４１％，说明其对着丝粒蛋白有较强的损害作用。同时，染色体中期相分析表明，ＧＭＡ的染色体断裂作用较强，并且染色体损伤主要集中在着丝粒区域 In this paper, the centromere signal ratio induced by the compound glycidylmethacrylate (GMA) was detected by fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining (CREST) , To determine GMA aneuploidy induced effect. The results showed that about 40.9-57  5% of micronuclei induced by GMA were FISH positive, indicating that it has some aneuploidy toxicity, but the positive CREST was only 17  0-241%, indicating that the opposite Thyroglobulin has a strong damaging effect. Meanwhile, metaphase analysis of chromosomes showed that chromosome cleavage of GMA was stronger, and chromosome damage mainly concentrated in the centromere region