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近年来,随着免疫组化技术的发展和各种特异性抗体的出现,使许多疑难肿瘤病例得到了明确诊断。在常规肿瘤病理诊断中,5%~10%的病例仅靠HE染色难以做出明确的形态学诊断,这就要依靠免疫组化检测来帮助鉴别,尤其在低分化或未分化肿瘤的鉴别诊断中准确率可达50%~75%[1]。在免疫组化染色前,抗原热修复几乎对所有被检测的标记物而言必不可少,因为在组织固定过程中甲醛会与蛋白质发生交联反应,形成一种亚甲基桥交联结构,从而使组织中抗原决定簇被屏蔽。石善溶教授发明的抗原热修复可以破坏甲醛固定引起的蛋白交联,使被屏蔽的抗原决定簇重新暴露出来,从而能与相应抗体结合[2]。我科在多年免疫组化操作过程中发现,并非所有抗体通
In recent years, with the development of immunohistochemical techniques and the emergence of a variety of specific antibodies, many difficult cases of cancer have been diagnosed. In conventional pathological diagnosis of tumors, 5% to 10% of cases by HE staining is difficult to make a clear morphological diagnosis, which will rely on immunohistochemical detection to help identify, especially in poorly differentiated or undifferentiated tumor differential diagnosis In the accuracy of up to 50% to 75% [1]. Prior to immunohistochemical staining, antigenic thermo-repair is essential for almost all of the markers tested because formalin reacts with the protein during tissue fixation to form a crosslinked methylene bridge, Thus the antigenic determinants in the tissue are shielded. The thermal repair of antigen invented by Professor Shi Shanrui can destroy the cross-linking of proteins caused by formaldehyde fixation and make the shielded epitope re-exposed, so that it can bind with the corresponding antibodies [2]. Our department found in many years of immunohistochemical operation, not all antibodies pass