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适配体(Aptamers)是通过指数富集的配体系统进化(systematic evolution of ligands by exponential enrichment,SELEX)技术,从随机核酸文库中筛选出来的单链寡核苷酸,已在临床医疗及其他领域得到日益广泛的应用.与抗体相比,适配体具有很多优点,如高亲和力、高特异性、分子量小、几乎无免疫排斥反应、结构稳定、易于合成等.可用于适配体筛选的靶标范围非常广,包括有机小分子、蛋白质、完整细胞及病毒颗粒等.迅速可靠的病原检测对于病毒性传染病的成功预防和治疗具有重要意义.随着严格筛选和快速分离技术的进步,适配体在病毒感染的检测治疗中显示出巨大的潜力.本文概括介绍了适配体在病毒研究方面的最新应用进展及未来前景.
Aptamers are single-stranded oligonucleotides that have been screened from random nucleic acid libraries by systematic evolution of ligands by exponential enrichment (SELEX) technology and have been used in clinical and other The field has been more and more widely used.Compared with antibodies, aptamers have many advantages, such as high affinity, high specificity, small molecular weight, almost no immune rejection, structural stability, ease of synthesis, etc. Can be used for aptamer screening The target range is very wide, including small organic molecules, proteins, intact cells and virus particles, etc. Rapid and reliable pathogen detection is of great significance for the successful prevention and treatment of viral infectious diseases.With the strict screening and rapid separation technology advances, the appropriate Ligands have shown great potential in the detection and treatment of viral infections.In this paper, the latest application and future of aptamers in virus research are summarized.