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目的研究喂饲转HJC-1和G6-EPSPS基因抗虫耐草甘膦大米对大鼠的亚慢性毒性。方法将140只健康6～8周龄SPF级Wistar大鼠按体重随机分成7组,分别为饲养对照组和低(17.5%)、中(35%)、高(70%)剂量转基因大米组及低(17.5%)、中(35%)、高(70%)剂量亲本组,每组20只,雌雄各半。采用饲喂方式染毒,连续饲养90 d。观察大鼠的一般情况,测定体重、进食量;在实验中期(第45天)和实验结束时(第90天)检测血液学和血生化指标,在实验结束后测定各主要脏器重量,计算脏器系数;并进行脏器病理组织学检查。结果各剂量组动物的体重均呈增长趋势,转基因组、亲本对照组与饲养对照组每周体重及体重总增重、每周进食量、总食物利用率以及中、末期的血液学和血生化指标,各主要脏器系数及病理组织学检查比较,均未发现有生物学意义的改变。结论未发现喂饲转HJC-1和G6-EPSPS基因抗虫耐草甘膦大米对大鼠具有亚慢性毒性。
Objective To study the subchronic toxicity of glyphosate-tolerant rice fed HJC-1 and G6-EPSPS on rats. Methods 140 healthy Wistar rats aged 6-8 weeks old were randomly divided into 7 groups according to body weight, which were fed control group and low (17.5%), medium (35%) and high (70%) dose of genetically modified rice Low (17.5%), medium (35%), high (70%) dose of the parent group, each group of 20, male and female. The use of feeding poisoning, continuous feeding 90 d. The general condition of the rats was observed, the body weight and food intake were measured, the hematological and blood biochemical indexes were measured in the middle of the experiment (day 45) and the end of the experiment (day 90), and the weight of each major organ was measured after the experiment Organ coefficient; and organ histopathological examination. Results The weight of animals in each dose group showed an increasing tendency. The body weight, body weight gain, weekly food intake, total food utilization rate, mid and late hematology and blood biochemistry in transgenic group, parental control group and feeding control group Indicators, the major organ coefficient and histopathological examination, no biological changes were found. CONCLUSION No sub-chronic toxicity was found in rats fed the transgenic HJC-1 and G6-EPSPS transgenic insect-resistant glyphosate-tolerant rice.