Aim:To study the antiviral effect of Oenanthejavanica flavones(OjF)on humanhepatoma HepG2.2.15 culture system and duck hepatitis B virus(DHBV)infection.Methods:(1)After incubation for 24 h,the 2.2.15 cells were treated with differentconcentrations of OjF for 12 d.The cell alteration was observed by microscope.The presence of HBsAg and HBeAg were measured using the enzyme immunoas-say kit after 2.2.15 cells were treated with OjF for 9d.(2)Ducklings infected withDHBV intravenously were divided into 5 groups and treated with OjF,acyclovir(ACV),and normal saline respectively for 10 d.All the ducklings were bled before,during,and after treatments at different times,and serum levels of DHBV-DNAwere detected by a dot-blot hybridization assay.Results:(1)The 50% toxicconcentration(TC_(50))of OjF was 2.28 g/L.The maximum nontoxic concentration(TC_0)was 1.00 g/L.In nontoxic concentrations,OjF significantly inhibited HBsAgand HBeAg in 2.2.15 cells after 9 d of treatment(P<0.05,P<0.01).(2)The DHBV-DNA levels decreased significantly alter the treatment with 0.50 and 1.00g/kg ofOjF(P<0.01).The inhibition of the peak of viremia was maximum at a dose of 1.00g/kg and reached 54.3% on d 5 and 64.5% on d 10,respectively.Conclusion:Theresults demonstrate that OjF is a strong inhibitor of HBsAg and HBeAg secretionin 2.2.15 cells and DHBV-DNA levels in the HBV-infected duck model. Aim: To study the antiviral effect of Oenanthejavanica flavones (OjF) on human hepatpatovirus HepG2.2.15 culture system and duck hepatitis B virus (DHBV) infection. Methods: (1) After incubation for 24 h, the 2.2.15 cells were treated with different concentrations of OjF for 12 days. The cell alteration was observed by microscope. The presence of HBsAg and HBeAg were measured using the enzyme immunoas-say kit after 2.2.15 cells were treated with OjF for 9d. (2) Ducklings infected with DDHV intravenously were divided into 5 groups and treated with OjF, acyclovir (ACV), and normal saline respectively for 10 d. All the ducklings were bled before, during, and after treatments at different times, and serum levels of DHBV-DNAwere detected by a dot-blot Hybridization assay. Results: (1) The 50% toxicconcentration (TC_ (50)) of OjF was 2.28 g / L.The maximum nontoxic concentration (TC_0) was 1.00 g / L.In nontoxic concentrations, OjF significantly inhibited HBsAg and HBeAg in 2.2 .15 cells after 9 days of treatment (P <0.05, P <0.01). (2) The DHBV- DNA inhibition of the peak of viremia was at a dose of 1.00 g / kg and reached 54.3% on d 5 and 64.5% on d (P <0.01) 10, respectively.Conclusion: Theresults demonstrate that OjF is a strong inhibitor of HBsAg and HBeAg secretionin 2.2.15 cells and DHBV-DNA levels in the HBV-infected duck model.