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自1965年Pluznik和Sachs创立了造血细胞体外软琼脂培养技术以来真.对集落刺激因子(CSP)的研究也逐渐增多。本文报道了小鼠脾脏细胞体外集落形成的方法,并对人尿中CSF以及外周血白细胞培养和小鼠脾脏细胞培养中获得的条件性培养液(CM)刺激集落生长的活力进行了比较。
Since the establishment of hematopoietic cell soft agar culture in vitro by Pluznik and Sachs in 1965, the research on colony stimulating factor (CSP) has also gradually increased. This article reports the method of colony formation of mouse spleen cells in vitro and compared the CSF activity in human urine and the activity of conditioned medium (CM) stimulated colony growth in peripheral blood leukemia cells and mouse spleen cells.