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【目的】观察冰黄五苓散直肠滴注对大脑中动脉缺血(MCAO)大鼠脑组织S100B蛋白、神经元特异性烯醇化酶(NSE)表达的影响。【方法】选用63只健康雄性SD大鼠随机分为治疗组、假手术组和模型组,根据处死时间每组再随机分为3 d、7 d、14 d 3个亚组。对各组大鼠进行神经功能缺损评分,采用干—湿质量法行脑组织含水量测定,采用免疫组织化学法检测大鼠脑组织中S100B、NSE表达。【结果】治疗组大鼠神经功能缺损评分明显低于模型组,差异有统计学意义(P<0.05);治疗组大鼠脑组织含水量、S100B的表达低于模型组,而NSE的表达高于模型组,差异均有统计学意义(P<0.05)。【结论】冰黄五苓散具有改善MCAO大鼠神经功能缺损症状、减轻脑水肿、保护大脑神经功能作用。
【Objective】 To observe the effects of Binghuang Wuling scattered rectal infusion on the expression of S100B protein and neuron-specific enolase (NSE) in brain of middle cerebral artery occlusion rats (MCAO). 【Methods】 Sixty-three healthy male Sprague-Dawley rats were randomly divided into treatment group, sham-operation group and model group. According to the time of death, each group was randomly divided into three subgroups: 3 d, 7 d and 14 d. The neurological deficit scores of rats in each group were determined. The water content of brain tissue was determined by dry-wet method. The expression of S100B and NSE in brain tissue of rats were detected by immunohistochemical method. 【Results】 The score of neurological deficit in the treatment group was significantly lower than that of the model group (P <0.05). The water content of the brain tissue in the treatment group was lower than that in the model group, while the expression of NSE was high In the model group, the differences were statistically significant (P <0.05). 【Conclusion】 Bing Huang Wuling San decoction can improve symptoms of neurological deficits, reduce brain edema and protect neurological function in MCAO rats.