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目的通过分析2005-2009年度脉动真空蒸汽灭菌效果监测结果,初步探讨影响灭菌效果的因素,找出灭菌失败的原因,不断持续改进监测技术,提高灭菌效果。方法采用物理、化学和生物方法对脉动真空蒸汽灭菌效果进行监测。结果 2005-2007年度B-D试验合格率为97.8%,化学监测合格率为98.3%,生物监测合格率为99.7%,灭菌物品监测合格率为100%。引进标准测试包法,改进监测方法后,2008-2009年度B-D试验合格率为99.5%,化学监测合格率为99.4%,生物监测合格率为100%,灭菌物品监测合格率为100%。结论 3种监测方法监测过程及结果均未达到全部合格,B-D试验和化学监测受人为因素和设备因素影响较多,应加强管理,定期检修灭菌设备,严格操作规程,提高监测技术,确保物品灭菌质量。
Objective By analyzing the monitoring results of pulsating vacuum steam sterilization in 2005-2009, the factors influencing the sterilization effect were initially discussed, the reasons of sterilization failure were found, and the monitoring technique was continuously improved to improve the sterilization effect. Methods The effects of pulsating vacuum steam sterilization were monitored by physical, chemical and biological methods. Results The pass rate of B-D test in 2005-2007 was 97.8%, the pass rate of chemical monitoring was 98.3%, the pass rate of biological monitoring was 99.7% and the pass rate of sterilized articles was 100%. After introducing the standard test package method and improving the monitoring method, the pass rate of B-D test in 2008-2009 was 99.5%, the pass rate of chemical monitoring was 99.4%, the pass rate of biological monitoring was 100% and the pass rate of sterilized articles monitoring was 100%. Conclusion The monitoring methods and results of the three monitoring methods did not meet all the requirements. BD test and chemical monitoring are more affected by human factors and equipment factors. Management should be strengthened and the sterilization equipment should be regularly checked and repaired. Strict operation procedures should be followed to improve the monitoring technology to ensure that the items Sterilization quality.