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以猴头菇为试材,采用磷酸缓冲液(PBS)浸提法制备提取液,在浸提剂pH值、料液比、浸提时间、浸提温度等单因素试验的基础上,采用正交实验设计,研究了猴头菇凝集素的最佳提取工艺。结果表明:最佳的提取工艺以PBS(0.10 mol/L,pH 6.5)为提取剂,料液比1∶15(g/mL),提取温度4℃,提取时间24h。在此条件下,猴头菇凝集素的凝集活性达到0.586,提取率达到1.35%。
Hericium erinaceus was used as test material, and the extract was prepared by phosphate buffered saline (PBS) extraction. Based on the single factor experiments such as the pH value of extract, the ratio of solid to liquid, extraction time and extraction temperature, The experimental design, to study the best extraction of Hericium lectin. The results showed that the optimal extraction conditions were as follows: PBS (0.10 mol / L, pH 6.5) as extractant, solid-liquid ratio 1:15 (g / mL), extraction temperature 4 ℃ and extraction time 24h. Under these conditions, the agglutination activity of Hericium lectin reached 0.586, the extraction rate reached 1.35%.