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研究了药材甲(Stegobium paniceum)、烟草甲(Lasioderma serricorne)和咖啡豆象(Araecerus fasciculatus)3种中药材储藏期害虫谷胱甘肽转移酶的酶活性,探讨了该酶在CO2气调胁迫下的应激反应。结果表明,药材甲、烟草甲和咖啡豆象的谷胱甘肽转移酶比活力分别为(0.339 2±0.015 5)(、0.966 0±0.259 3)nmol/(min.mg)和(0.370 2±0.032 8)nmol/(min.mg)。CO2气调对3种昆虫GSTs的影响研究结果表明,在CO2气调处理3、6、9 h时,药材甲、烟草甲和咖啡豆象成虫的GSTs酶比活力均有一定程度的升高。可见,CO2气调亚致死处理对3种实验昆虫GSTs有一定的诱导激活作用。研究结果对于储藏物害虫气调杀虫机制的分析有指导意义。
The enzyme activities of glutathione transferase (GSH) in three kinds of Chinese herbal medicines such as Stegobium paniceum, Lasioderma serricorne and Araecerus fasciculatus during storage were studied. Stress response. The results showed that the specific activities of glutathione transferase in Radix Aconiti, Nicotiana A and Caffeine were respectively (0.339 2 ± 0.015 5) (0.966 ± 0.259 3) nmol / (min.mg) and (0.370 2 ± 0.032 8) nmol / (min.mg). The effects of CO2 on the GSTs of three species of insectsThe results showed that the GSTs’ specific activity increased when treated with CO2 atmosphere for 3, 6, and 9 h. It can be seen that CO2-mediated sub-lethal treatment induced certain activation of GSTs in three experimental insects. The research results are instructive for the analysis of insecticidal mechanism of stored-pests insects and insects.